The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
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Details-enriched characterization of cell populations. Morphological information and facts from photographs can increase to the richness of flow cytometry data. By way of example, the determine shows an in any other case standard apoptosis assay working with Annexin V and PI, adding cell imaging to characterize cells in Each individual inhabitants to expose morphologically distinct capabilities. These insights couldn't are gained from multiplex staining on your own.
As samples are acquired over the Attune CytPix Flow Cytometer, the high-velocity brightfield digital camera captures and outlets pictures of detected activities in a level nearly six,000 illustrations or photos for each 2nd, according to the flow charge and impression dimension. For increased versatility, Attune Cytometric Software package allows you to modify the picture capture frequency as desired and choose a specific gate for picture capture, if essential.
(B) RAW 264.seven macrophages tend to be annotated inaccurately with respect to particle rely because of their complexity and noncircular borders. The quantity of singlets accessible for Investigation increases after the consumer trains and employs a more accurate product.
Discover new insights into your experimental knowledge by visualizing populations applying blended fluorescence and morphology data. Our computer software interface wizards permit downsampling, concatenation (merged information) and dimensionality resources that are built into your program for an intuitive, streamlined information Evaluation practical experience.
"A picture is not fairly worth 1000 dots, but it surely actually helps you to see images. It is excellent to view pictures that correlate with staining. Such as, AnnexinV-beneficial cells that are morphologically distinct than healthful cells, or CD14+ monocytes that happen to be larger sized and more textured than CD3+/CD4+/CD14- lymphocytes.
Listed here, a skilled user has gated singlets confidently. Just after assessing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate consists of more than four% aggregates.
Pair your Attune flow cytometer with an autosampler for increased workflow performance, and add robotic integration for maximum throughput.
Our researchers will style panels for yourself as often times you require—free of charge, no product or service obtain required.
The Attune NxT cytometer will be the trusted workhorse instrument your lab can rely on for constant and dependable overall performance. The Attune CytPix model includes a higher-velocity brightfield digicam and AI-pushed graphic analysis software program, with latest updates such as device Mastering applications and an intuitive person interface, enabling Improved details precision, precision, and more quickly insights into sample biology.
End users can look at occasion photographs in authentic-time to verify sample excellent, improve protocols, and history the morphology of each and every cell inhabitants recognized for Investigation. Imaging capture also Advantages from acoustic focusing, which really helps to place the cells making sure that a pointy, centered picture is obtained.
Sound waves empower exact cell alignment, leading to exceptional details good quality and ~10X quicker sample flow prices.
Application Notice: Automated graphic Investigation decreases person-to-user variability in get more info flow cytometry gating methods
Leverage the strength of Sophisticated technologies and innovation to speed up your discoveries. Belief while in the Attune Flow Cytometer spouse and children to deliver the dependability, pace, and precision that established your work aside.
No clean/no lyse leukocyte Assessment with imaging. Cells ended up acquired from 24-hour-old blood by dilution in one mM EDTA ( Expert UX/UI permits buyers to coach AI styles for expanded apps. (A) The “Teach Model” characteristic presents annotation instruments and genuine-time design coaching precision.
Gating on these prolonged parameters means that you can speedily and properly recognize populations of curiosity and ensure gating tactic with little if any guide review. The Attune program features use of condition-of-the-art equipment Discovering analysis applications within an expertly designed user interface, rendering it exceptionally user friendly.
The Attune Xenith cytometer is a substantial-parameter spectral analyzer, offering equally spectral unmixing and conventional flow abilities for in depth and functional cell analysis.
The Attune CytPix Flow Cytometer enables consumers to visualize samples through acquisition, validate the accuracy of gating tactics, and help outcomes with trustworthy documentation for publication functions.
Here, we exhibit that by gating tactics employing picture-primarily based quantitative parameters can distinguish interacting cells from coincident activities to much more accurately examine interacting cells.